Characters played by Tobey Maguire
Peter Parker
Spider-Man
Characters played by Alfred Molina
Dr. Otto Octavius
Doc Ock
Characters played by Kirsten Dunst
Mary Jane Watson
Characters played by Bruce Campbell
Tour Guide
Characters played by Holly Fields
Black Cat
Characters played by Joe Alaskey
Dr. Kurt Connors
Characters played by Josh Keaton
Harry Osborn
Additional Voices
Characters played by Jay Gordon
J. Jonah Jameson
Additional Voices
Characters played by Charles Klausmeyer
John Jameson
Additional Voices
Characters played by Bethany Rhoades
Betty Brant
Additional Voices
Characters played by Jeff Coopwood
Robbie Robertson
Additional Voices
Characters played by Mindy Sterling
Aunt May
Additional Voices
Characters played by John DiMaggio
Rhino
Characters played by James Arnold Taylor
Quentin Beck
Mysterio
Additional Voices
Characters played by Michael Beattie
Shocker
Additional Voices
Characters played by Keith Szarabajka
Mr. Azizi
Additional Voices
Characters played by Mike McColl
Police Officer
Characters played by Larry Huffman
Arena Announcer
Characters played by Angela Shelton
Calypso
Additional Voices
Characters played by Rocky Carroll
Additional Voices
Characters played by Peter Lurie
Additional Voices
Characters played by Niecy Nash
Additional Voices
Characters played by David Sobolov
Shocker Henchman
Jewel Thief
Characters played by Herschel Sparber
Additional Voices
Characters played by Tasia Valenza
Additional Voices
Characters played by Dee Bradley Baker
The Puma
Mysterio
MysterioBot
Train Conductor
Bank Teller
Scientist
Characters played by Susan Egan
Rosie Octavius
Characters played by Loren Lester
Dr. Parker
Additional Voices
Characters played by Colleen O'Shaughnessey
Characters played by Gary Anthony Sturgis
Luke Cage
Additional Voices
Characters played by Al Collado
Cab Driver