Characters played by Kevin Conroy
Bruce Wayne
Batman
Characters played by Mark Hamill
The Joker
Dr. Isaac Evers
Characters played by Arleen Sorkin
Dr. Harleen Quinzel
Harley Quinn
'Mary Flynn'
Characters played by Tara Strong
Barbara Gordon
Batgirl
Characters played by Michael Ansara
Dr. Victor Fries
Mr. Freeze
Characters played by Diane Pershing
Dr. Pamela Isley
Poison Ivy
Characters played by Efrem Zimbalist Jr.
Alfred Pennyworth
Characters played by Bob Hastings
Commissioner Jim Gordon
Characters played by Lloyd Bochner
Mayor Hamilton Hill