Characters played by Dimitra Arliss
Anastasia Hardy
Characters played by Sara Ballantine
Mary Jane Watson
Characters played by Christopher Daniel Barnes
Spider-Man
Peter Parker
Characters played by Bob Bergen
Additional Voices
Characters played by Roscoe Lee Browne
Kingpin
Wilson Fisk
Characters played by Scott Cleverdon
Michael Pingree
Characters played by Stephanie Eustase
Maria Taina Elizando
Characters played by Linda Gary
Aunt May Parker
Characters played by David Hayter
Additional Voices
Characters played by Adam Hendershott
Additional Voices
Characters played by Theresa Hill
Additional Voices
Characters played by Glen Hopkins
Additional Voices
Characters played by Alan Johnson
Additional Voices
Characters played by Joan Lee
Madame Web
Characters played by Anthony Marciona
Additional Voices
Characters played by Anne Moss
Additional Voices
Characters played by David Paladino
Additional Voices
Characters played by Marla Rubinoff
Liz Allen
Characters played by Michael Rye
Dr. Farley Stillwell
Characters played by Rodney Saulsberry
Robbie Robertson
Characters played by Cannon Young
Additional Voices
Characters played by Efrem Zimbalist Jr.
Dr. Octopus
Dr. Otto Octavius