Characters played by Ogie Banks
Clayface
Mr. Freeze
Characters played by Steve Blum
Batman
Joker
Killer Moth
Two-Face
Killer Croc
Characters played by Chris Edgerly
Mad Hatter
Man-Bat
Characters played by Keith Ferguson
Alfred Pennyworth
Commissioner Gordon
Characters played by Grey Griffin
Harley Quinn
Batgirl
Characters played by Tom Kenny
Riddler
Penguin
Characters played by Vanessa Marshall
Poison Ivy
Catwoman
Characters played by Fred Tatasciore
Bane
Hush
Characters played by James Arnold Taylor
Robin
Nightwing
Characters played by Dave Wittenberg
Scarecrow