Characters played by Frank Welker
Scooby-Doo
Fred Jones
Characters played by Matthew Lillard
Shaggy Rogers
Characters played by Grey Griffin
Daphne Blake
Characters played by Kate Micucci
Velma Dinkley
Characters played by Cassandra Peterson
Elvira
Mistress of the Dark
Characters played by Bill Nye
Bill Nye
Characters played by Dwight Schultz
Dr. Jonathan Crane
Scarecrow
Characters played by Kamali Minter
Michelle
Characters played by Bumper Robinson
Mike
Characters played by Roger Craig Smith
Autopilot
Parent
Characters played by Fred Tatasciore
Alpha Jackal Lantern
Federal Agent Malarkey
Characters played by David Herman
Sheriff
Cutler Toe
Characters played by Phyllis Diller
Self