Characters played by Kazuya Nakai
Toshiro Hijikata
Characters played by Kenichi Suzumura
Sogo Okita
Characters played by Susumu Chiba
Isao Kondo
Characters played by Tomokazu Sugita
Gintoki Sakata
Characters played by Daisuke Sakaguchi
Shinpachi Shimura
Characters played by Rie Kugimiya
Kagura
Characters played by Kenta Miyake
Shachi
Characters played by Norio Wakamoto
Katakuriko Matsudaira
Characters played by Marina Inoue
Asaemon Ikeda
Characters played by Urara Takano
Princess Bubbles
Characters played by Fumihiko Tachiki
Taizo Hasegawa
Characters played by Yukari Tamura
Ana Hanano
Characters played by Show Hayami
Umibozu
Characters played by Shin'ichirô Miki
Tatsuma Sakamoto
Characters played by Kujira
Otose
Characters played by Akeno Watanabe
Mutsu
Characters played by Satsuki Yukino
Tae Shimura
Characters played by Fumiko Orikasa
Kyubei Yagyu
Characters played by Binbin Takaoka
Former Tendoshu Leader
Characters played by Yû Kobayashi
Ayame Sarutobi
Characters played by Yûko Kaida
Tsukuyo