Characters played by Samuel L. Jackson
Afro Samurai
Ninja Ninja
Characters played by Phil LaMarr
Brother 1
Brother 3
Brother 5
Kuro
Teen Afro Samurai
Characters played by Yuri Lowenthal
Kuma
Jinno
Characters played by Greg Eagles
Brother 6
Afro's Dad
Brother #6
Characters played by Terrence 'T.C.' Carson
Sword Master
Brother #4
Swordmaster
Characters played by John DiMaggio
Brother 2
Brother #2
Giant
Ivanov
Patron 2
Characters played by Ron Perlman
Justice
Characters played by S. Scott Bullock
Dharman
Characters played by Fred Tatasciore
Juzo
Patron 5
Shuzo
Characters played by Dave Wittenberg
Assassin
Matasaburo
Patron 3
Punk
Characters played by Jeff Bennett
Foo
Hachiro
Characters played by Jason Marsden
Sasuke
Characters played by Tara Strong
Otsuru
Jiro
Characters played by Crystal Scales
Young Afro
Young Afro Samurai
Characters played by Kelly Hu
Okiku
Characters played by Steve Blum
Assassin
Characters played by Grey Griffin
Oyuki
Woman
Characters played by John Kassir
Soshun
Characters played by Liam O'Brien
Kihachi
Patron 4
Characters played by Dwight Schultz
Assassin 1
Patron 1
Ronin
Characters played by James Arnold Taylor
Yashichi